By M. P. O’Connell, U. Priefer, N. Datta, M. E. Nugent (auth.), Alfred Pühler, Kenneth N. Timmis (eds.)
The improvement of robust new strategies and refmements of tech niques in molecular genetics in recent times, and the surge in curiosity in biotechnology in line with genetic tools, have heralded a brand new golden age in molecular genetics, and motivated in varied disciplines a lot curiosity within the applied sciences themselves and their strength makes use of in uncomplicated and utilized biomedical sciences. even though a few very good professional laboratory manuals (especially the chilly Spring Harbor Laboratory manuals through I. H. Miller; R. W. Davies et al. ; and T. Maniatis et al. ) on sure chapters of molecular genetics exist, no normal textual content that covers a extensive spectrum of the sub ject has up to now been released. the aim of this guide is to pre despatched so much, even though of necessity no longer all the vital equipment of molecular genetics, in a chain of straightforward experiments, a lot of which might be without problems entire via the microbiologist, biochemist or biotechnologist that has had simply restricted publicity to genetics. the rest of the experiments require both better familiarity with the topic, or assistance through a person with such event. The ebook may still, consequently, not just let members to procure new proce dures for ongoing tasks, but additionally function a foundation for the instructing of molecular genetic thoughts in formal predoctoral and postdoctoral laboratory courses.
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Extra resources for Advanced Molecular Genetics
Coli chromosome. Due to lack of suitable conjugation systems, many bacterial strains of scientifIc or commercial importance could not be analyzed. More recently, a plasmid was isolated by Haas and Holloway (1976) , which is able to mobilize the bacterial chromosome in Pseudomonas aeruginosa. 45 is a derivative of R68, which confers resistance to ampicillin, kanamycin, and tetracycline. It is self-transmissable and belongs to the incompatibility group P. 45 lies in its ability to promote host chromosome transfer (ema) in a very extensive range of Gram-negative bacteria following conjugation (Holloway 1979), unlike the F plasmid.
D) Materials. . . . . . . . . . . . . . . . . . .. 7. References. . . . . . . . . . . . . . . . . . . . . . 38 39 39 39 39 40 40 40 40 41 44 45 45 46 46 47 47 47 48 48 49 50 1. General Introduction The experiments described below characterize antibiotic resistance plasmids that will replicate in E. coli K12. For other plasmids and plasmids of other bacterial species, methods must be adapted. No general instructions are possible for the characterization of any plasmid in any bacterial genus, although the availability of a plasmid-free I Dept.
2. Experiment 1: Mobilization of Host Chromosomal Genes in E. coli. a) Introduction. . . . . . . . . . . . . . . . b) Objectives. . . . . . . . . . . . . . . . c) Procedure . . . . . . . . . . . . . . . . d) Results. . . . . . . . . . . . . . . . . 3. Experiment 2: Mobilization of E. coli Vector Plasmids. . . . . a) Introduction. . . . . . . . . . . . . . . . b) Objectives. . .
Advanced Molecular Genetics by M. P. O’Connell, U. Priefer, N. Datta, M. E. Nugent (auth.), Alfred Pühler, Kenneth N. Timmis (eds.)