Download e-book for iPad: Advances in Disease Vector Research by Alexander S. Raikhel (auth.), Kerry F. Harris (eds.)

By Alexander S. Raikhel (auth.), Kerry F. Harris (eds.)

ISBN-10: 1461229103

ISBN-13: 9781461229100

ISBN-10: 1461277167

ISBN-13: 9781461277163

Volume nine during this sequence contains 4 chapters on vectors that impact human or animal future health and 6 chapters on plant pathogens and their vectors. In bankruptcy 1, Alex S. Raikhel discusses vitellogenesis in mosquitoes: the cornerstone of the reproductive cycle related to vast construction of yolk precursors via the fats physique and their accumulation in constructing oocytes. In anautogeneous mosquitoes, vitellogenesis depends at the availability of a blood meal and, consequently, is associated with transmission of pathogens. for that reason, elucidation of mechanisms governing the mosquito vitellogenesis is necessary for the profitable improvement of novel concepts in vector and sickness administration. earlier stories on mosquito vitellogenesis have dealt predominantly with hormonal regulate. The objective of this evaluate, even though, is to summarize major growth which has been accomplished in knowing mosquito vitellogenesis on the mobile, biochemical and molecular degrees. it's with those disciplines that we predict to completely comprehend the mechanisms governing this key procedure in mosquito reproduction.

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32 Alexander S. Raikhel References 1. , J. Cell BioI. 50:201-221. 2. , 1978, Purification and partial characterization of Culex fatigans yolk protein, Insect Biochem. 8:111-115. 3. , 1985, Tyrosine sulfation of yolk proteins 1,2, and 3 in Drosophila melanogaster. J. Bioi. Chem. 260:6434-6439. 4. , 1983, Mosquito juvenile hormone: identification and bioassay activity, J. Insect Physiol. 29:465470. 5. , 1980, Induction of follicle separation in the mosquito by physiological amounts of ecdysterone, Science Washington, 209:819-821.

9. Visualization of the mosquito Vg receptor by the ligand-blotting technique. Membranes from ovaries 24 hrs PBM were solubilized with octyl-beta-Dglucoside and separated on 6% SDS polyacrylamide gel under reducing (lane 1) and non-reducing (lane 2) conditions followed by transfer to a nitrocellulose membrane. Ligand blotting was performed by incubating the nitrocellulose membrane with 150,000 cpm/ml of [ 35 S]Vg. Binding of radiolabeled Vg to its receptor was visulized by autoradiography of the washed and dried blot.

As demonstrated by the analysis of microsomal fractions, the high-molecular weight Vg precusor (pro-Vg) exists as an intermediate in the biosynthesis of Vg. However, this precursor could be detected in whole fat body extracts only during the first few hours after the initiation of Vg synthesis by normal blood-feeding. 4A). It is likely that 20-HE effects only transcription of Vg genes, which results in accumulation of pro-Vg. 1. Effect of 20-hydroxyecdysone on vitellogenin synthesis by fat bodies from previtellogenic mosquitoes cultured in vitro.

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Advances in Disease Vector Research by Alexander S. Raikhel (auth.), Kerry F. Harris (eds.)

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